Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum.
Identifieur interne : 000A20 ( Main/Exploration ); précédent : 000A19; suivant : 000A21Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum.
Auteurs : Leila Kalai-Grami [Tunisie] ; Sabrine Saidi ; Sarra Bachkouel ; Imen Ben Slimene ; Monia Mnari-Hattab ; Mohamed Rebah Hajlaoui ; Ferid LimamSource :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2014.
English descriptors
- KwdEn :
- Ascomycota, Bacillus (genetics), Bacillus (isolation & purification), Bacillus (metabolism), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Lipopeptides (genetics), Lipopeptides (metabolism), Peptide Synthases (genetics), Peptide Synthases (metabolism), Plant Diseases, Random Amplified Polymorphic DNA Technique.
- MESH :
- chemical , genetics : Bacterial Proteins, Lipopeptides, Peptide Synthases.
- genetics : Bacillus.
- isolation & purification : Bacillus.
- metabolism : Bacillus, Bacterial Proteins, Lipopeptides, Peptide Synthases.
- Ascomycota, Plant Diseases, Random Amplified Polymorphic DNA Technique.
Abstract
A collection of 200 bacterial isolates recovered from citrus plants (Citrus limon, Citrus sinensis, and Citrus reticulata), Medicago truncatula and Laurus nobilis, was established. In vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens Phoma tracheiphila and Verticillium albo-atrum. Isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (PGPB) abilities, as well as for the presence of nonribosomal peptide synthetase (NRPS) genes responsible of the lipopeptide biosynthesis. The results were positive for 16 isolates which exhibited at least two PGPB activities and a single NRPS gene. Genetic diversity of the selected isolates was studied using random amplified polymorphic DNA (RAPD) and repetitive element PCR (REP) tools that showed clustering of strains into three major groups (I, II, and III) (i, ii, and iii), respectively. Clustering was further confirmed by the 16S rDNA sequencing that assigned nine isolates to Bacillus velezensis, four isolates to Bacillus methyltrophicus, one isolate to Bacillus amyloliquefaciens, and two isolates to Bacillus mojavensis. Organ-bacterial genotype interaction as well as positive correlation with NRPS genes are discussed.
DOI: 10.1007/s12010-014-1062-4
PubMed: 25074353
Affiliations:
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Bachkouel</name></author><author><name sortKey="Ben Slimene, Imen" sort="Ben Slimene, Imen" uniqKey="Ben Slimene I" first="Imen" last="Ben Slimene">Imen Ben Slimene</name></author><author><name sortKey="Mnari Hattab, Monia" sort="Mnari Hattab, Monia" uniqKey="Mnari Hattab M" first="Monia" last="Mnari-Hattab">Monia Mnari-Hattab</name></author><author><name sortKey="Hajlaoui, Mohamed Rebah" sort="Hajlaoui, Mohamed Rebah" uniqKey="Hajlaoui M" first="Mohamed Rebah" last="Hajlaoui">Mohamed Rebah Hajlaoui</name></author><author><name sortKey="Limam, Ferid" sort="Limam, Ferid" uniqKey="Limam F" first="Ferid" last="Limam">Ferid Limam</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25074353</idno><idno type="pmid">25074353</idno><idno type="doi">10.1007/s12010-014-1062-4</idno><idno type="wicri:Area/PubMed/Corpus">000301</idno><idno type="wicri:Area/PubMed/Curation">000301</idno><idno 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Karray, 2049, Tunis</wicri:regionArea><wicri:noRegion>Tunis</wicri:noRegion></affiliation></author><author><name sortKey="Saidi, Sabrine" sort="Saidi, Sabrine" uniqKey="Saidi S" first="Sabrine" last="Saidi">Sabrine Saidi</name></author><author><name sortKey="Bachkouel, Sarra" sort="Bachkouel, Sarra" uniqKey="Bachkouel S" first="Sarra" last="Bachkouel">Sarra Bachkouel</name></author><author><name sortKey="Ben Slimene, Imen" sort="Ben Slimene, Imen" uniqKey="Ben Slimene I" first="Imen" last="Ben Slimene">Imen Ben Slimene</name></author><author><name sortKey="Mnari Hattab, Monia" sort="Mnari Hattab, Monia" uniqKey="Mnari Hattab M" first="Monia" last="Mnari-Hattab">Monia Mnari-Hattab</name></author><author><name sortKey="Hajlaoui, Mohamed Rebah" sort="Hajlaoui, Mohamed Rebah" uniqKey="Hajlaoui M" first="Mohamed Rebah" last="Hajlaoui">Mohamed Rebah Hajlaoui</name></author><author><name sortKey="Limam, Ferid" sort="Limam, Ferid" uniqKey="Limam F" first="Ferid" last="Limam">Ferid Limam</name></author></analytic><series><title level="j">Applied biochemistry and biotechnology</title><idno type="eISSN">1559-0291</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota</term><term>Bacillus (genetics)</term><term>Bacillus (isolation & purification)</term><term>Bacillus (metabolism)</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Lipopeptides (genetics)</term><term>Lipopeptides (metabolism)</term><term>Peptide Synthases (genetics)</term><term>Peptide Synthases (metabolism)</term><term>Plant Diseases</term><term>Random Amplified Polymorphic DNA Technique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Lipopeptides</term><term>Peptide Synthases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacillus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacillus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacillus</term><term>Bacterial Proteins</term><term>Lipopeptides</term><term>Peptide Synthases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Ascomycota</term><term>Plant Diseases</term><term>Random Amplified Polymorphic DNA Technique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A collection of 200 bacterial isolates recovered from citrus plants (Citrus limon, Citrus sinensis, and Citrus reticulata), Medicago truncatula and Laurus nobilis, was established. In vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens Phoma tracheiphila and Verticillium albo-atrum. Isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (PGPB) abilities, as well as for the presence of nonribosomal peptide synthetase (NRPS) genes responsible of the lipopeptide biosynthesis. The results were positive for 16 isolates which exhibited at least two PGPB activities and a single NRPS gene. Genetic diversity of the selected isolates was studied using random amplified polymorphic DNA (RAPD) and repetitive element PCR (REP) tools that showed clustering of strains into three major groups (I, II, and III) (i, ii, and iii), respectively. Clustering was further confirmed by the 16S rDNA sequencing that assigned nine isolates to Bacillus velezensis, four isolates to Bacillus methyltrophicus, one isolate to Bacillus amyloliquefaciens, and two isolates to Bacillus mojavensis. Organ-bacterial genotype interaction as well as positive correlation with NRPS genes are discussed.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Bachkouel, Sarra" sort="Bachkouel, Sarra" uniqKey="Bachkouel S" first="Sarra" last="Bachkouel">Sarra Bachkouel</name><name sortKey="Ben Slimene, Imen" sort="Ben Slimene, Imen" uniqKey="Ben Slimene I" first="Imen" last="Ben Slimene">Imen Ben Slimene</name><name sortKey="Hajlaoui, Mohamed Rebah" sort="Hajlaoui, Mohamed Rebah" uniqKey="Hajlaoui M" first="Mohamed Rebah" last="Hajlaoui">Mohamed Rebah Hajlaoui</name><name sortKey="Limam, Ferid" sort="Limam, Ferid" uniqKey="Limam F" first="Ferid" last="Limam">Ferid Limam</name><name sortKey="Mnari Hattab, Monia" sort="Mnari Hattab, Monia" uniqKey="Mnari Hattab M" first="Monia" last="Mnari-Hattab">Monia Mnari-Hattab</name><name sortKey="Saidi, Sabrine" sort="Saidi, Sabrine" uniqKey="Saidi S" first="Sabrine" last="Saidi">Sabrine Saidi</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Kalai Grami, Leila" sort="Kalai Grami, Leila" uniqKey="Kalai Grami L" first="Leila" last="Kalai-Grami">Leila Kalai-Grami</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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